Cell–free Protein Synthesis: Methods and Protocols - Hardcover

Hardcover

ISBN 10: 3527316493 ISBN 13: 9783527316496

Publisher: Wiley VCH, 2007

Synopsis

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

About this title

About the Author

Alexander Spirin is a Professor at the Moscow State University, and the director of the Protein Research Institute of the Russian Academy of Sciences in Pushchino, Russia. He is one of the pioneers in the biochemical analysis of protein synthesis and has worked in the field for more than 40 years, performing the first-ever in vitro synthesis of proteins by using ribosome components. Professor Spirin has received many honors for his scientific achievements, including the Hans Adolf Krebs Medal, and is the author of several books on ribosomes and protein synthesis. James Swartz is Professor of Chemical Engineering at Stanford University, California, USA. A graduate of the Massachusetts Institute of Technology, he has pioneered protein synthesis on an industrial scale while working for the companies Eli Lilly and Genentech. He is a leading expert on the biotechnological synthesis of mammalian proteins using bacterial whole cells and cell extracts.

From the Back Cover

Complex biomolecules such as proteins cannot simply be synthesized by standard chemical reactions. They need to be assembled making use of the biological mechanisms that generate them in the living cell. Recreating the biosynthesis of proteins in a controlled cell-free laboratory system is no trivial task, yet brings enormous benefits in terms of product cost and purity that are particularly relevant for biotechnological applications.
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, this practical handbook covers the latest advances in the field -- both on the analytical and the preparative scale.
Following an introduction, the book goes on to deal with the constructive approach for cell-free translation, recombinant human proteins, and cell-free expression and production. It also covers novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering, as well as gene cloning and expression in molecular colonies.
The step-by-step protocols and practical examples given for each method represent practical advice for beginners and experts alike.

Edition details

• Publisher: Wiley VCH
• Publication date: 2007
• ISBN 10: 3527316493
• ISBN 13: 9783527316496
• Binding: Hardcover
• Language: English
• Edition number: 1
• Number of pages: 262
• Editor: Spirin Alexander S., Swartz James R.