Multi-Parametric Live Cell Microscopy of 3D Tissue Models - Softcover

Softcover

ISBN 10: 3319673599 ISBN 13: 9783319673592

Publisher: Springer, 2017

Synopsis

Current state-of-the-art 3D tissue models and their compatibility with live cell imaging.- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation.- Quantitative live cell FLIM imaging in three dimensions.- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview.- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues.- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture.- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2.- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell.- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues.- Live cell imaging of viscosity in 3D tumour cell models.- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy.- Raman imaging microscopy for quantitative analysis of biological samples.

Edition details

• Publisher: Springer
• Publication date: 2017
• Language: English
• ISBN 10: 3319673599
• ISBN 13: 9783319673592
• Binding: Paperback
• Number of pages: 186
• Editor: Dmitriev Ruslan I.