Items related to A Laboratory Guide to Biotin-Labeling in Biomolecule...
Synopsis
2.1 Introduction ...32 2.2 Technical Procedures ...32 Chemical Synthesis ...35 2.3 Results and Discussion...38 ...Chemical Synthesis ...38 Binding to anti-CCK antisera and CCK-B receptors ...40 Binding to streptavidin agarose and photoelution ...40 Affinity chromatography ...41 2.4 Troubleshooting...43 References ...44 ...Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ...45 Summary ...45 3.1 Introduction ...46 ...3.2 Technical Procedures ...48 Preparation of a Biotinylated Ligand ...48 Preparation of Membrane Fraction ...50 Solubilization of the PACAP Receptor ...51 Partial Purification of the PACAP Receptor ...52 Affinity Purification of the PACAP Receptor ...54 Final Purification of the PACAP Receptor ...56 Receptor-Binding Assay ...57 3.3 Results and Discussion...58 ...3.4 Troubleshooting ...61 Impurity in PACAP27-Cys-NH...61 2 Low specific activity of the PACAP receptor despite a single band ...62 Acknowledgments ...62 References ...63 ...Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ..." 65 Summary ...65 4.1 Introduction...66 . 4.2 Technical Procedures ..." 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ...67 Synthesis of photo reactive biotinylated peptide hormones ...67 Site-specific incorporation of biotin and photo labels in separate steps ...69 Photoaffinity labeling ...72 4.3 Results and Discussion ...76 Synthesis of photoactivatable insulins with permanent biotin labels ...76 Applications: Insulin ...78 VI Examples for other applications ...78 4.4 Troubleshooting...79 References ...81 ...
"synopsis" may belong to another edition of this title.
Search results for A Laboratory Guide to Biotin-Labeling in Biomolecule...
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (eng)
Print on DemandSeller: Brook Bookstore On Demand, Napoli, NA, Italy
Seller rating 5 out of 5 stars
Condition: new. Questo è un articolo print on demand. Seller Inventory # 6STC9K2H8W
Buy New
£ 39.68
£ 4.58 shipping
Ships from Italy to U.S.A.
Ships from Italy to U.S.A.
Quantity: Over 20 available
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Print on DemandSeller: Chiron Media, Wallingford, United Kingdom
Seller rating 5 out of 5 stars
PF. Condition: New. This item is printed on demand. Brand new book, sourced directly from publisher. Dispatch time is 6-7 days from our warehouse. Book will be sent in robust, secure packaging to ensure it reaches you securely. Seller Inventory # 6666-IUK-9783034873512
Buy New
£ 47.76
£ 4.99 shipping
Ships from United Kingdom to U.S.A.
Ships from United Kingdom to U.S.A.
Quantity: 20 available
Seller Image
Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Seller: GreatBookPrices, Columbia, MD, U.S.A.
Seller rating 5 out of 5 stars
Condition: New. Seller Inventory # 20183802-n
Seller Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Published by
Springer, Basel, Birkhäuser Basel, Birkhäuser Mrz 2012, 2012
ISBN 10: 3034873514
ISBN 13: 9783034873512
New
Taschenbuch
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch. Seller Inventory # 9783034873512
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Seller: Books Puddle, Woodside, NY, U.S.A.
Seller rating 4 out of 5 stars
Condition: New. pp. 260. Seller Inventory # 26128410826
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
Seller: Revaluation Books, Exeter, United Kingdom
Seller rating 5 out of 5 stars
Paperback. Condition: Brand New. 1996 edition. 260 pages. 9.61x6.69x0.59 inches. In Stock. Seller Inventory # x-3034873514
Buy New
£ 66.24
£ 10 shipping
Ships from United Kingdom to U.S.A.
Ships from United Kingdom to U.S.A.
Quantity: 2 available
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Print on DemandSeller: Majestic Books, Hounslow, United Kingdom
Seller rating 4 out of 5 stars
Condition: New. Print on Demand pp. 260 67:B&W 6.69 x 9.61 in or 244 x 170 mm (Pinched Crown) Perfect Bound on White w/Gloss Lam. Seller Inventory # 131128085
Buy New
£ 72.59
£ 6.50 shipping
Ships from United Kingdom to U.S.A.
Ships from United Kingdom to U.S.A.
Quantity: 4 available
Seller Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Print on DemandSeller: moluna, Greven, Germany
Seller rating 5 out of 5 stars
Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- The Avidin-Biotin Interaction.- Avidin.- Streptavidin.- Biotin.- 1.2 Technical Procedures.- Determining Biotin and Biotin Incorporation Levels.- Biotinylation R. Seller Inventory # 4319092
Buy New
£ 41.52
£ 40.83 shipping
Ships from Germany to U.S.A.
Ships from Germany to U.S.A.
Quantity: Over 20 available
Stock Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Print on DemandSeller: Biblios, Frankfurt am main, HESSE, Germany
Seller rating 4 out of 5 stars
Condition: New. PRINT ON DEMAND pp. 260. Seller Inventory # 18128410816
Seller Image
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Seller: AHA-BUCH GmbH, Einbeck, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . Seller Inventory # 9783034873512