Items related to A Laboratory Guide to Biotin-Labeling in Biomolecule...
Synopsis
2.1 Introduction ...32 2.2 Technical Procedures ...32 Chemical Synthesis ...35 2.3 Results and Discussion...38 ...Chemical Synthesis ...38 Binding to anti-CCK antisera and CCK-B receptors ...40 Binding to streptavidin agarose and photoelution ...40 Affinity chromatography ...41 2.4 Troubleshooting...43 References ...44 ...Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ...45 Summary ...45 3.1 Introduction ...46 ...3.2 Technical Procedures ...48 Preparation of a Biotinylated Ligand ...48 Preparation of Membrane Fraction ...50 Solubilization of the PACAP Receptor ...51 Partial Purification of the PACAP Receptor ...52 Affinity Purification of the PACAP Receptor ...54 Final Purification of the PACAP Receptor ...56 Receptor-Binding Assay ...57 3.3 Results and Discussion...58 ...3.4 Troubleshooting ...61 Impurity in PACAP27-Cys-NH...61 2 Low specific activity of the PACAP receptor despite a single band ...62 Acknowledgments ...62 References ...63 ...Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ..." 65 Summary ...65 4.1 Introduction...66 . 4.2 Technical Procedures ..." 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ...67 Synthesis of photo reactive biotinylated peptide hormones ...67 Site-specific incorporation of biotin and photo labels in separate steps ...69 Photoaffinity labeling ...72 4.3 Results and Discussion ...76 Synthesis of photoactivatable insulins with permanent biotin labels ...76 Applications: Insulin ...78 VI Examples for other applications ...78 4.4 Troubleshooting...79 References ...81 ...
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Springer, Basel, Birkhäuser Basel, Birkhäuser Mrz 2012, 2012
ISBN 10: 3034873514
ISBN 13: 9783034873512
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch. Seller Inventory # 9783034873512
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . Seller Inventory # 9783034873512
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- The Avidin-Biotin Interaction.- Avidin.- Streptavidin.- Biotin.- 1.2 Technical Procedures.- Determining Biotin and Biotin Incorporation Levels.- Biotinylation R. Seller Inventory # 4319092
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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ISBN 10: 3034873514
ISBN 13: 9783034873512
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -ceptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .Springer Basel AG in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 260 pp. Englisch. Seller Inventory # 9783034873512
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