Structural and Biochemical Characterization of the YaxAB Pore-forming Toxin from Yersinia Enterocolitica (Springer Theses) - Hardcover

Bräuning, Bastian

Hardcover

ISBN 10: 3030294382 ISBN 13: 9783030294380

Publisher: Springer, 2019

Synopsis

This book discusses the decoding of the lytic mechanism of an α-helical pore-forming toxin, YaxAB, composed of two different subunits. Pore-forming toxins (PFTs) are among the most common bacterial toxins. They are produced by a variety of pathogens, which infect a wide range of organisms including plants, insects and humans. Yet the maturation of these particles and the structural changes required for pore formation are still poorly understood for many PFT families.

 

Using a diverse panel of biochemical and structural techniques, including X-ray crystallography and cryo-electron microscopy, Dr. Bräuning and colleagues have succeeded in identifying the mechanistic contributions of the two toxin components and elucidating the lytic state of the pore complex. The results of this thesis on the YaxAB system are applicable to orthologues from agriculturally relevant insect pathogens, and offer valuable structural and mechanistic insights to inform future bioengineering efforts. 

About this title

About the Author

Bastian Bräuning completed his undergraduate and postgraduate education at the LMU Munich and the Weizmann Institute of Science, Israel. He subsequently pursued doctoral training with Prof. Michael Groll at the TUM Munich, where he mastered biochemical and structural techniques for the analysis of membrane protein complexes. Currently, he is a postdoctoral fellow in Prof. Brenda Schulman’s group at the Max Planck Institute of Biochemistry, where his focus continues to be on challenging membrane protein targets and their regulation. 

From the Back Cover

This book discusses the decoding of the lytic mechanism of an α-helical pore-forming toxin, YaxAB, composed of two different subunits. Pore-forming toxins (PFTs) are among the most common bacterial toxins. They are produced by a variety of pathogens, which infect a wide range of organisms including plants, insects and humans. Yet the maturation of these particles and the structural changes required for pore formation are still poorly understood for many PFT families. 

Using a diverse panel of biochemical and structural techniques, including X-ray crystallography and cryo-electron microscopy, Dr. Bräuning and colleagues have succeeded in identifying the mechanistic contributions of the two toxin components and elucidating the lytic state of the pore complex. The results of this thesis on the YaxAB system are applicable to orthologues from agriculturally relevant insect pathogens, and offer valuable structural and mechanistic insights to inform future bioengineering efforts. 

Edition details

• Publisher: Springer
• Publication date: 2019
• Language: English
• ISBN 10: 3030294382
• ISBN 13: 9783030294380
• Binding: Hardcover
• Edition number: 1
• Number of pages: 86