PCR: Methods Express - Softcover

Simon Hughes; Adrian Moody

Softcover

ISBN 10: 1904842283 ISBN 13: 9781904842286

Publisher: Scion Publishing Ltd, 2007

Synopsis

PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR *rapid generation of gene targeting constructs *PCR multiplexes *PCR-based mutagenesis *identification of microdeletions and microduplications *DNA methylation analysis *forensic genetic DNA typing *genotyping *identification of mutations in single cells *whole genome amplification *diagnosis of infectious diseases *inverse PCR-based RFLP This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.

About this title

From the Publisher:

This title is part of Scion's Methods Express series: a series of books
written for busy life science researchers. Each book provides: protocols in
step-by-step detail; comprehensive troubleshooting; example data as
benchmarks; key references and further reading.
Books in the series include:
Bioinformatics (190484216X), Biosensors (1904842127), Cell Imaging
(1904842046), DNA Microarrays (1904842151), Immunohistochemistry
(1904842038), PCR (1904842283), Proteomics (1904842135), Whole Genome
Amplification (1904842070)

Synopsis:

PCR is probably the most widely-used technique in molecular biology today, with new variants appearing on a frequent basis. Many of the new PCR methods offer substantial benefits over protocols that have previously been covered in original papers and lab manuals. "PCR: Methods Express" describes the latest PCR-based methodologies and approaches and provides the most up-to-date practical advice on how to tackle a broad range of biological problems using these techniques, including: quantitative RT-PCR; identification of genomic micro-deletions and duplications; DNA methylation analysis; DNA typing for forensic genetics; rapid generation of gene targeting constructs; pre-implantation genetic disease diagnosis; construction of long DNA molecules from multiple fragments; and identification of low level mutations in tumors. "PCR: Methods Express" is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategies for optimization.

This book is an essential laboratory manual for researchers in all biomedical fields, and at all levels, from postgraduate student to principal investigator.

About this edition

• Publisher: Scion Publishing Ltd
• Publication date: 2007
• ISBN 10: 1904842283
• ISBN 13: 9781904842286
• Binding: Paperback
• Edition number: 1
• Number of pages: 348
• Editor: Simon Hughes, Adrian Moody