Proteomics: Methods Express (Life Science) - Hardcover

C. David O'Connor; B. David Hames

Hardcover

ISBN 10: 1904842208 ISBN 13: 9781904842200

Publisher: Scion Publishing Ltd, 2007

Synopsis

Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong. Contents 1. Sample preparation and subcellular fractionation approaches: purification of membranes and their microdomains for mass spectrometry analysis (Yan Li , Phil Oh, and Jan E. Schnitzer, Sidney Kimmel Cancer Center); 2. An isotope-coding strategy for quantitative proteomics (Xian Chen, Dept of Biochemistry and Biophysics, University of N. Carolina at Chapel Hill); 3. Gel-based approaches (Stuart Cordwell and Ben Crossett, both at School of Molecular and Microbial Biosciences, University of Sydney, and Melanie Y. White, Minomic Pty Ltd); 4. Peptide sorting by reverse-phase diagonal chromatography (Kris Gevaert and Joel Vandekerckhove, Faculty of Medicine and Health Sciences, Ghent University); 5. Mass spectrometry strategies for protein identification (David R. Goodlett, University of Washington and Garry L. Corthals, University of Turku); 6. Desorption electrospray ionization: proteomics studies by a method that bridges ESI and MALDI (Zoltan Takats, Justin M. Wiseman, Demian R. Ifa and R.Graham Cooks, all at Dept of Chemistry, Purdue University); 7. Analysis of cellular protein complexes by affinity purification and mass spectrometry (Tilmann Buerckstuemmer and Keiryn L. Bennett, both at Research Center for Molecular Medicine of the Austrian Academy of Sciences); 8. Clinical proteomic profiling and disease signatures (Rosamonde E. Banks, David A. Cairns, David N. Perkins and Jennifer H. Barrett, all at Cancer Research UK Clinical Centre, St James' s University Hospital, Leeds); 9. Characterization of post-translational modifications: undertaking the phosphoproteome (W. Andy Tao, Purdue University; Bernd Bodenmiller and Ruedi Aebersold, both Institute for Molecular Systems Biology, Federal Institute of Technology, Zurich); 10. Protein microarray technologies (Chien-Sheng Chen, Sheng-Ce Tao, and Heng Zhu, Dept of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine); 11. Intelligent mining of complex data: challenging the proteomic bottleneck (Dan Bach Kristensen, Maxygen and Alexandre Potelejnikov, Proxeon); 12. Bioinformatic approaches in proteomics (Sandra Orchard and Henning Hermjakob, both European Bioinformatics Institute, Hinxton); List of suppliers; Index. From the Publisher This title is part of Scion's Methods Express series: a series of books written for busy life science researchers. Each book provides: protocols in step-by-step detail; comprehensive troubleshooting; example data as benchmarks; key references and further reading. Books in the series include: Bioinformatics (9781904842163), Biosensors (9781904842125), Cell Imaging (9781904842040), DNA Microarrays (9781904842156), Expression Systems (9781904842439), Immunohistochemistry (9781904842033), PCR (9781904842286), Proteomics (9781904842132), Whole Genome Amplification (9781904842071).

About this title

From the Publisher

This title is part of Scion's Methods Express series: a series of books
written for busy life science researchers. Each book provides: protocols in
step-by-step detail; comprehensive troubleshooting; example data as
benchmarks; key references and further reading.
Books in the series include:
Bioinformatics (190484216X), Biosensors (1904842127), Cell Imaging
(1904842046), DNA Microarrays (1904842151), Immunohistochemistry
(1904842038), PCR (1904842283), Proteomics (1904842135), Whole Genome
Amplification (1904842070)

Synopsis

Conventional approaches to proteomics have been augmented with a new generation of technologies unfamiliar to many life science researchers. "Proteomics: Methods Express" identifies the most powerful of these new technologies and presents them in a way that allows their robust implementation in even non-specialist labs. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and currently cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. (Deleted contents since listed below again - and in greater detail) This book is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success - and troubleshooting for when things go wrong. "Proteomics: Methods Express" is an essential laboratory manual for researchers in all biomedical fields and at all levels, from postgraduate student to principal investigator.

Edition details

• Publisher: Scion Publishing Ltd
• Publication date: 2007
• Language: English
• ISBN 10: 1904842208
• ISBN 13: 9781904842200
• Binding: Hardcover
• Number of pages: 272
• Editor: C. David O'Connor, B. David Hames