This book covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but is equally relevant to the imaging of fixed specimens. Contents 1. An introduction to cell imaging (David Stephens); 2. Confocal or wide-field? A guide to selecting appropriate methods for cell imaging (Mark A. Jepson); 3. Image quantification and analysis (Kota Miura and Jens Rietdorf); 4. Transmitted light imaging (Jon D. Lane and Howard Stebbing); 5. Spectral imaging techniques for fluorescence microscopy (Timo Zimmermann); 6. Measurement of protein motion by photobleaching (John F. Presley); 7. Imaging calcium and calcium-binding proteins (Burcu Hasdemir, Robert Burgoyne and Alexei Tepikin); 8. Total internal reflection fluorescence microscopy (David Zenisek); 9. An overview of three-dimensional and four-dimensional microscopy by computational deconvolution (Jose-Angel Conchello); 10. Correlative microscopy using Tokuyasu cryosections: applications for immunolabeling and in situ hybridization (Miguel R. Branco, Sheila Q. Xie, et al.); 11. Fluorescence lifetime imaging (Klaus Suhling); d 12. Homo-FRET measurements to investigate molecular-scale organization of proteins in living cells (Rajat Varma and Satyajit Mayor); 13. High-content and high-throughput screening (Elizabeth P. Roquemore); List of suppliers; Index. From the Publisher: This title is part of Scion's Methods Express series: a series of books written for busy life science researchers. Each book provides: protocols in step-by-step detail; comprehensive troubleshooting; example data as benchmarks; key references and further reading. Books in the series include: Bioinformatics (9781904842163), Biosensors (9781904842125), Cell Imaging (9781904842040), DNA Microarrays (9781904842156), Expression Systems (9781904842439), Immunohistochemistry (9781904842033), PCR (9781904842286), Proteomics (9781904842132), Whole Genome Amplification (9781904842071).
"synopsis" may belong to another edition of this title.
' ... a lot of distilled wisdom is clearly presented throughout for the immediate benefit of beginner or experienced microscopist alike. I wholeheartedly recommend it, advise you to buy it and expect my own copy to become a well-thumbed friend.' --RMS Proceedings
This title is part of Scion's Methods Express series: a series of books
written for busy life science researchers. Each book provides: protocols in
step-by-step detail; comprehensive troubleshooting; example data as
benchmarks; key references and further reading.
Books in the series include:
Bioinformatics (190484216X), Biosensors (1904842127), Cell Imaging
(1904842046), DNA Microarrays (1904842151), Immunohistochemistry
(1904842038), PCR (1904842283), Proteomics (1904842135), Whole Genome
Amplification (1904842070)
"About this title" may belong to another edition of this title.
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