Real-Time PCR: Current Technology and Applications - Hardcover

Hardcover

ISBN 10: 1904455395 ISBN 13: 9781904455394

Publisher: Caister Academic Press Limited, 2013

Synopsis

Real-time PCR Real-time PCR (RT-PCR) technology is highly flexible and many alternative instruments and fluorescent probe systems have been developed recently. The decreased hands-on time, increased reliability, and improved quantitative accuracy of RT-PCR methods have contributed to the adoption of RT-PCR for a wide range of new applications. This essential manual presents a comprehensive guide to the most up- Full description

About this title

Product Description:

Essential manual providing a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique.

Excerpt. © Reprinted by permission. All rights reserved.:

The development of instruments that allowed real-time monitoring of fluorescence within PCR reaction vessels was a significant advance. The technology is flexible and many alternative instruments and fluorescent probe systems have been developed and are currently available. Real-time PCR assays can be completed rapidly since no manipulations are required post-amplification. Identification of the amplification products by probe detection in real-time is highly accurate compared with size analysis on gels. Analysis of the progress of the reaction allows accurate quantification of the target sequence over a very wide dynamic range, provided suitable standards are available. Further investigation of the real-time PCR products within the original reaction mixture using probes and melting analysis can detect sequence variants including single base mutations. Since the first practical demonstration of the concept real-time PCR has found applications in many branches of biological science. Applications include gene expression analysis, the diagnosis of infectious disease and human genetic testing. Due to their fluorimetry capabilities, these real-time machines are also compatible with alternative amplification methods such as NASBA, provided a fluorescence end-point is available.

About this edition

• Publisher: Caister Academic Press Limited
• Publication date: 2013
• ISBN 10: 1904455395
• ISBN 13: 9781904455394
• Binding: Hardcover
• Edition number: 1
• Number of pages: 302
• Editor: Logan J