Detecting Pathogens in Food (Woodhead Publishing Series in Food Science, Technology and Nutrition) - Hardcover

T.A. McMeekin

Hardcover

ISBN 10: 1855736705 ISBN 13: 9781855736702

Publisher: Woodhead Publishing Ltd, 2003

Synopsis

Methods for detecting pathogens need to balance accuracy, specificity and speed. There have been major advances in techniques in all these areas. Detecting pathogens in food sums up the wealth of recent research, the range of techniques available and their applications. The first part of the book looks at general issues such as the role of microbiological analysis in food safety management, sampling techniques and ways of validating individual detection methods. The second part of the book examines individual techniques, including microscopy, immunological and genetic methods.

Key Features: reviews the latest techniques in microbiological analysis and how they can best be used to ensure food safety; examines the role of microbiological analysis in food safety management and discusses the range of detection techniques available; includes chapters on electrical methods, ATP bioluminescence, microscopy techniques and immunological methods such as ELISAs.

Contents: Part 1 General issues: Microbiological analysis and food safety management; GMP and HACCP systems; Sampling techniques; Separation and concentration of samples; Validating detection techniques; Quality assurance of laboratory performance. Part 2 Particular techniques: Culture methods; Electrical methods; ATP bioluminescence; Microscopy techniques: DEFT and flow cytometry; Immunological techniques: immunochromatography, enzyme linked immunofluorescent assays and agglutination techniques; Immunological techniques: ELISA; Genetic techniques: PCR, NASBA, hybridisation and microarrays; Genetic techniques: molecular subtyping methods; New biosensors for microbiological analysis; The use of applied systematics in identifying foodborne pathogens.

About this title

About the Author

Professor Tom McMeekin is Director of the Centre for Food Safety and Quality at the University of Tasmania, Australia.

From the Inside Flap

Identifying pathogens in food quickly and accurately is one of the most important requirements in food processing. The ideal detection method needs to combine such qualities as sensitivity, specificity, speed and suitability for on-line applications. Detecting pathogens in food brings together a distinguished international team of contributors to review the latest techniques in microbiological analysis and how they can best be used to ensure food safety.

Part 1 looks at general issues, beginning with a review of the role of microbiological analysis in food safety management. There are also chapters on the critical issues of what to sample and how samples should be prepared to make analysis effective, as well as how to validate individual detection techniques and assure the quality of analytical laboratories.

Part 2 discusses the range of detection techniques now available, beginning with traditional culture methods. There are chapters on electrical methods, ATP bioluminescence, microscopy techniques and the wide range of immunological methods such as ELISAs. Two chapters look at the exciting developments in genetic techniques, the use of biosensors and applied systematics.

Detecting pathogens in food is a standard reference for all those concerned in ensuring the safety of food.

Edition details

• Publisher: Woodhead Publishing Ltd
• Publication date: 2003
• Language: English
• ISBN 10: 1855736705
• ISBN 13: 9781855736702
• Binding: Hardcover
• Number of pages: 384
• Editor: T.A. McMeekin