Quantitative Proteomics: Rsc: Volume 1 (New Developments in Mass Spectrometry) - Hardcover

Hardcover

ISBN 10: 1849738084 ISBN 13: 9781849738088

Publisher: Royal Society of Chemistry, 2014

Synopsis

This exemplary book will be essential reading for analytical and biological mass spectrometrists working in proteomics research, as well as those undertaking either fundamental or clinical-based investigations with an interest in understanding protein dynamics and/or biomarker assessment.

About this title

From the Back Cover

As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses focussed initially on relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of the problem being addressed.

Quantitative Proteomics aims to outline the state of the art in mass spectrometry-based quantitative proteomics, describing recent advances and current limitations in the instrumentation used, together with the various methods employed for generating high quality data. Details on both strategies describing how stable isotope labelling can be applied and methods for performing quantitative analysis of proteins in a label-free manner are given. The utility of these strategies to understanding cellular protein dynamics are then exemplified with chapters looking at spatial proteomics, dynamics of protein function as determined by quantifying changes in protein post-translational modification and protein turnover. Finally, a key application of these techniques to biomarker discovery and validation is presented, together with the rapidly developing area of quantitative analysis of protein-based foodstuffs.

This exemplary book is essential reading for analytical and biological mass spectrometrists working in proteomics research, as well as those undertaking either fundamental or clinical-based investigations with an interest in understanding protein dynamics and/or biomarker assessment.

From the Inside Flap

As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses focussed initially on relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of the problem being addressed.

Quantitative Proteomics aims to outline the state of the art in mass spectrometry-based quantitative proteomics, describing recent advances and current limitations in the instrumentation used, together with the various methods employed for generating high quality data. Details on both strategies describing how stable isotope labelling can be applied and methods for performing quantitative analysis of proteins in a label-free manner are given. The utility of these strategies to understanding cellular protein dynamics are then exemplified with chapters looking at spatial proteomics, dynamics of protein function as determined by quantifying changes in protein post-translational modification and protein turnover. Finally, a key application of these techniques to biomarker discovery and validation is presented, together with the rapidly developing area of quantitative analysis of protein-based foodstuffs.

This exemplary book is essential reading for analytical and biological mass spectrometrists working in proteomics research, as well as those undertaking either fundamental or clinical-based investigations with an interest in understanding protein dynamics and/or biomarker assessment.

Excerpt. © Reprinted by permission. All rights reserved.

Quantitative Proteomics

By Claire Eyers, Simon J Gaskell

The Royal Society of Chemistry

Copyright © 2014 The Royal Society of Chemistry
All rights reserved.

ISBN: 978-1-84973-808-8

Contents

Technology, 
Chapter 1 Practical Considerations and Current Limitations in Quantitative Mass Spectrometry-based Proteomics Adam M. Hawkridge, 
Chapter 2 High Resolution/Accurate Mass Targeted Proteomics A. Bourmaud, S. Gallien and B. Domon, 
Label-based Protein Quantification, 
Chapter 3 Making Sense Out of the Proteome: the Utility of iTRAQ and TMT Narciso Couto, Caroline A. Evans, Jagroop Pandhal, Wen Qiu, Trong K. Pham, Josselin Noirel and Phillip C. Wright, 
Chapter 4 Getting Absolute: Determining Absolute Protein Quantities via Selected Reaction Monitoring Mass Spectrometry Christina Ludwig and Ruedi Aebersold, 
Chapter 5 Proteomics Standards with Controllable Trueness — Absolute Quantification of Peptides, Phosphopeptides and Proteins Using ICP- and ESI-MS Anna Konopka, Christina Wild, Martin E. Boehm and Wolf D. Lehmann, 
Label-free Protein Quantification, 
Chapter 6 Overview and Implementation of Mass Spectrometry-Based Label-Free Quantitative Proteomics Erik J. Soderblom, J. Will Thompson and M. Arthur Moseley, 
Chapter 7 MS1 Label-free Quantification Using Ion Intensity Chromatograms in Skyline (Research and Clinical Applications) Birgit Schilling, Brendan X. MacLean, Alexandria D'Souza, Matthew J. Rardin, Nicholas J. Shulman, Michael J. MacCoss and Bradford W. Gibson, 
Chapter 8 Label-free Quantification of Proteins Using Data-Independent Acquisition Yishai Levin, 
Dynamic Protein Quantification, 
Chapter 9 Spatial Proteomics: Practical Considerations for Data Acquisition and Analysis in Protein Subcellular Localisation Studies Andy Christoforou, Claire Mulvey, Lisa M. Breckels, Laurent Gatto and Kathryn S. Lilley, 
Chapter 10 Quantitative Analyses of Phosphotyrosine Cellular Signaling in Disease Hannah Johnson, 
Chapter 11 Next Generation Proteomics: PTMs in Space and Time Dalila Bensadek, Armel Nicolas and Angus I. Lamond, 
Chapter 12 Experimental and Analytical Approaches to the Quantification of Protein Turnover on a Proteome-wide Scale Amy J. Claydon, Dean E. Hammond and Robert J. Beynon, 
Applications of Quantitative Proteomics, 
Chapter 13 Protein Quantification by MRM for Biomarker Validation L. Staunton, T. Clancy, C. Tonry, B. Hernández, S. Ademowo, M. Dharsee, K. Evans, A. C. Parnell, R. W. Watson, K. A. Tasken and S. R. Pennington, 
Chapter 14 MRM-based Protein Quantification with Labeled Standards for Biomarker Discovery, Verification, and Validation in Human Plasma Andrew J. Percy, Andrew G. Chambers, Carol E. Parker and Christoph H. Borchers, 
Chapter 15 Mass Spectrometry-based Quantification of Proteins and Peptides in Food Phil E. Johnson, Justin T. Marsh and E.N. Clare Mills, 
Subject Index, 

CHAPTER 1

Practical Considerations and Current Limitations in Quantitative Mass Spectrometry-based Proteomics

ADAM M. HAWKRIDGE

Departments of Pharmaceutics & Pharmacotherapy and Outcomes Sciences, Virginia Commonwealth University School of Pharmacy, Richmond, VA 23298, USA

* Email: amhawkridge@vcu.edu

1.1 Introduction

Mass spectrometry (MS)-based proteomics has become a prominent technology platform for quantitatively studying protein expression, modification, interaction, and degradation. Although more established protein quantification methods using antibodies, gel electrophoresis, and radiochemical labelling remain important in bio

Edition details

• Publisher: Royal Society of Chemistry
• Publication date: 2014
• Language: English
• ISBN 10: 1849738084
• ISBN 13: 9781849738088
• Binding: Hardcover
• Number of pages: 389
• Editor: Claire E Eyers