Recent Advances in Super-Resolution Microscopy Imaging - Hardcover

Aleksei Anatoliyovych Stepanenko (editor)

Hardcover

ISBN 10: 1773610325 ISBN 13: 9781773610320

Publisher: Delve Publishing, 2018

Synopsis

The fluorescence microscope is the principal tool for investigating the spatial organization of bio-molecular processes. Instrumentation and techniques have evolved considerably since the first realizations of fluorescence microscopy. The technology is constantly being advanced to image faster, deeper, less invasively, and more quantitatively, driving discovery of both biological and clinical mechanisms. In this book, we discuss the various approaches, which can be successfully used in live cells, the tradeoffs in resolution, speed, and ease of implementation, which one must make for each approach, and the quality of results that one might expect from each technique. We introduce the recent development of optical microscopy that has achieved spatial resolution beyond the diffraction limit. Super-resolution imaging techniques outperforming the classical diffraction limits are photoactivated localization microscopy (PALM), stochastic optical resolution microscopy (STORM), stimulated-emission depletion microscopy (STED), three-dimensional structured illumation microscopy (3D-SIM) and total internal reflection fluorescence microscopy (TIRF). These super-resolution microscopy techniques have permitted detailed studies of organelles, vesicles, microtubules, DNAs, proteins, and other biochemical particles on the nanometer scale. Super-resolution microscopy has opened the door to true nanoscale mapping of biological components.

About this title

About the Author

Aleksei A. Stepanenko received his Masters’s degree in Biochemistry from V. N. Karazin Kharkiv National University and PhD in Molecular biology from Institute of Molecular Biology and Genetics, where he is currently working as a Research Fellow. His scope of interests is genomic and phenotypic evolution of tumor cells under chemotherapeutic stresses and viro-immunological approaches as an alternative perspective in cancer therapy.

Edition details

• Publisher: Delve Publishing
• Publication date: 2018
• Language: English
• ISBN 10: 1773610325
• ISBN 13: 9781773610320
• Binding: Hardcover
• Number of pages: 275