Last four decades, with the increase in everlasting need there is still enormous advancements require in biological and chemical laboratory processes related to microfluidics devices. Microfluidics technology has found out plentiful uses at micro scale level e.g. flow cytometry, micro mixing, analysis of chemical and synthesis, particle sorting and generation of droplet. The use of various flow sensors and sensing systems at micro scale have drastically changed the scenario of higher end fields e.g. drug discovery, biomedical analyses, genetics, proteomics, biological and chemical reactions etc. Need of mixing is to take out high resolution and sensitive separations to distinguish very minute quantities of samples and reagents which makes this system comparatively inexpensive and requires short time analysis. The aim of the present work is to understand the physics of the mixing of fluids within micro channel using computational fluid dynamics and experimental technique. Four different types of mixing angles (300, 450, 600, 900) and three fluid mixing case of water+water; water+ethanol and water+glycerol were selected. The simulation results show that at 600 mixing angle minimum pressure drop and maximum outlet velocity was experienced as compared to other angles. At a constant inlet flow rate of 0.5 lph and at 600 degree angle the percentage variation between experimental and simulation results was observed to be in the range of 2.97-3.14% in water+water; 2.31-2.83% in water+ethanol and 19.62- 20.56% in water+glycerol case.
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