Protein-Protein Interactions (PPIs): Types, Methods for Detection & Analysis (Biochemistry Research Trends) - Hardcover

Clyde R Montgomery

Hardcover

ISBN 10: 1634859707 ISBN 13: 9781634859707

Publisher: Nova Science Publishers, Inc, 2016

Synopsis

Protein-protein interactions (PPIs) play an important role in vital biological processes such as metabolic and signaling pathways, cell cycle control, and DNA replication and transcription. This book discusses types, methods for detection and the analysis of PPIs. Chapter One describes two key nuclear magnetic resonance (NMR) methods for the investigation of protein-protein interactions, chemical shift perturbation, and residual dipolar coupling, with practical tips on the preparation of samples, NMR measurement, and analysis. Chapter Two describes the basic principles and provides a practical description of the recent technological developments in solution NMR spectroscopy for examining protein-protein interactions. Chapter Three focuses on identifying PPIs via many learning methods based on protein sequence information. Chapter Four examines the thermodynamics of PPIs by isothermal titration calorimetry. Chapter Five describes the general microscopic features of amyloid fibril structures and then discusses the macroscopic properties of protein aggregate with conformations such as packing, hydration, and enthalpy using thermodynamic variables in combination with densitometry and isothermal titration calorimetry. Chapter Six reviews the special case of protein self-association as a modulator of protein function.

Edition details

• Publisher: Nova Science Publishers, Inc
• Publication date: 2016
• Language: English
• ISBN 10: 1634859707
• ISBN 13: 9781634859707
• Binding: Hardcover
• Number of pages: 130