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Synopsis
“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
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From the Back Cover
“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
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Exon Skipping. Methods and Protocols
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Exon Skipping (eng)
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Exon Skipping: Methods and Protocols (Methods in Molecular Biology, 867)
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Exon Skipping
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -'Next generation' sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate 'real polymorphisms' from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping. 452 pp. Englisch. Seller Inventory # 9781617797668
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Exon Skipping
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Condition: New. This Methods in Molecular Biology(TM) book reviews methods and techniques used for exon skipping, such as methods on how to distinguish "real polymorphisms" from mutations that affect splicing. Includes protocols, materials lists, pitfalls and troubleshooting tips. Editor(s): Aartsma-Rus, Annemieke M. Series: Methods in Molecular Biology. Num Pages: 440 pages, 62 black & white illustrations, 8 colour illustrations, 15 black & white tables, biograph. BIC Classification: MFN; PSD. Category: (P) Professional & Vocational. Dimension: 254 x 178 x 29. Weight in Grams: 1037. . 2012. Hardback. . . . . Seller Inventory # V9781617797668
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Exon Skipping : Methods and Protocols
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Condition: Sehr gut. Zustand: Sehr gut | Seiten: 452 | Sprache: Englisch | Produktart: Bücher | ¿Next generation¿ sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate ¿real polymorphisms¿ from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping. Seller Inventory # 11619393/12
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Exon Skipping
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Buch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -¿Next generation¿ sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal manymutations that potentially lead to exon skipping. To functionally test thesea lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal modelsrequiring a more extensiveset of protocols.Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate ¿real polymorphisms¿ from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 452 pp. Englisch. Seller Inventory # 9781617797668