Items related to Pichia Protocols: 389 (Methods in Molecular Biology,...
Synopsis
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication.
"synopsis" may belong to another edition of this title.
Review
From the reviews of the second edition:
"P. pastoris has many superior traits. ... can grow to very high cell densities prior to switching on an inducible promoter (the AOX1 gene) for maximal recombinant protein production. The diversity of such proteins produced by expression strains of P. pastoris is quite amazing and this book describes detailed experimental protocols for a few of them (e.g. hookworm protein, botulinum toxin). ... This is a stand-alone book ... . a book that will prove valuable to yeast molecular geneticists and biotechnologists." (Graeme Walker, Microbiology Today, May, 2008)
From the Back Cover
The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system
"About this title" may belong to another edition of this title.
- PublisherHumana
- Publication date2010
- ISBN 10 1617375632
- ISBN 13 9781617375637
- BindingPaperback
- Number of pages280
- EditorCregg James M.
Other Popular Editions of the Same Title
Top Search Results from the AbeBooks Marketplace
Stock Image
Pichia Protocols (Methods in Molecular Biology, 389)
Seller: Lucky's Textbooks, Dallas, TX, U.S.A.
Seller rating 5 out of 5 stars
Condition: New. Seller Inventory # ABLIING23Mar2811580148414
Quantity: Over 20 available
Seller Image
Pichia Protocols (Methods in Molecular Biology) [Paperback ]
Seller: booksXpress, Bayonne, NJ, U.S.A.
Seller rating 4 out of 5 stars
Soft Cover. Condition: new. Seller Inventory # 9781617375637
Quantity: 3 available
Seller Image
Pichia Protocols
Published by
SPRINGER NATURE Nov 2010, 2010
ISBN 10: 1617375632
ISBN 13: 9781617375637
New
Taschenbuch
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition. 268 pp. Englisch. Seller Inventory # 9781617375637
Quantity: 2 available
Seller Image
Pichia Protocols
Print on DemandSeller: moluna, Greven, Germany
Seller rating 5 out of 5 stars
Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. All protocols are tested and readily and reproducibleUpdates and expands upon the first volumePresents the basics of Pichia pastoris and describes available vectors and host strains for expressionThis book focuses on rec. Seller Inventory # 458521428
Quantity: Over 20 available
Seller Image
Pichia Protocols
Published by
Springer Nature Singapore, 2010
ISBN 10: 1617375632
ISBN 13: 9781617375637
New
Taschenbuch
Seller: AHA-BUCH GmbH, Einbeck, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include adiscussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. Seller Inventory # 9781617375637
Quantity: 2 available
Stock Image
Pichia Protocols
Print on DemandSeller: Ria Christie Collections, Uxbridge, United Kingdom
Seller rating 5 out of 5 stars
Condition: New. PRINT ON DEMAND Book; New; Fast Shipping from the UK. No. book. Seller Inventory # ria9781617375637_lsuk
Quantity: Over 20 available
Stock Image
Pichia Protocols (Methods in Molecular Biology, 389)
Seller: Mispah books, Redhill, SURRE, United Kingdom
Seller rating 4 out of 5 stars
Paperback. Condition: Like New. Like New. book. Seller Inventory # ERICA77316173756326
Quantity: 1 available