Microarrays in Clinical Diagnostics: 114 (Methods in Molecular Medicine) - Softcover

Various, .

Softcover

ISBN 10: 1617375381 ISBN 13: 9781617375385

Publisher: Humana, 2010

Synopsis

Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic Analysis Yilmaz Niyaz, Monika Stich, Bernd Sägmüller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin Schütze Applications of the Universal DNA Microarray in Molecular Medicine Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis Barany Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization Zhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li, Qiong Zhang, and Jing Cheng Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays Lovisa Lovmar and Ann-Christine Syvänen Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, and Stefania Stenirri Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader® Margaret A. Keller Controlled Agitation During Hybridization: Surface Acoustic Waves Are Shaking Up Microarray Technology Achim Wixforth Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Regulator Gene by Luminex® xMAP™ Suspension Array Sherry A. Dunbar and James W. Jacobson Protein Microarray-Based Screening of Antibody Specificity Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, and Paul F. Predki Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection Brian B. Haab Allergen Microarrays Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, and Andrea Crisanti Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples Ruochun Huang, Qian Shi,Weimin Yang, and Ruo-Pan Huang Multiplexed Cytokine Sandwich Immunoassays: Clinical Applications Uma Prabhakar, Edward Eirikis, Bruce E. Miller, and Hugh M. Davis Validation and Quality Control of Protein Microarray-Based Analytical Methods Larry J. Kricka and Stephen R. Master Tissue Microarrays Ronald Simon, Martina Mirlacher, and Guido Sauter Index

About this title

From the Back Cover

Microarray technology is now well established in laboratories all over the world, both for rapid detection of genetic mutations and, increasingly, for simultaneously analyzing the expression of a large number of proteins. In Microarrays in Clinical Diagnostics, a panel of academic and industrial investigators surveys the new world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification and quantification of proteins, and for studies of protein-protein interactions. Among the highlights are universal arrays, antibody microarrays, direct labeling approaches, and quality control techniques for protein microarray-based analysis. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Microarrays in Clinical Diagnostics combines a comprehensive collection of cutting-edge technologies with detailed laboratory methods to help clinical laboratory researchers design and perform microarray experiments for DNA and protein analysis, diagnostic testing, and cancer research.

Edition details

• Publisher: Humana
• Publication date: 2010
• Language: English
• ISBN 10: 1617375381
• ISBN 13: 9781617375385
• Binding: Paperback
• Number of pages: 288
• Editor: Joos Thomas O., Fortina Paolo