PCR Mutation Detection Protocols: 688 (Methods in Molecular Biology, 688) - Hardcover

 
9781607619468: PCR Mutation Detection Protocols: 688 (Methods in Molecular Biology, 688)
Hardcover
ISBN 10: 1607619466 ISBN 13: 9781607619468
Publisher: Humana, 2010

Synopsis

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

"synopsis" may belong to another edition of this title.

From the Back Cover

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

"About this title" may belong to another edition of this title.

Publisher
Humana
Publication date
2010
Language
English
ISBN 10 
1607619466
ISBN 13 
9781607619468
Binding
Hardcover
Edition number
2
Number of pages
308
Editor
Theophilus Bimal D.M., Rapley Ralph

Other Popular Editions of the Same Title

9781493956296: PCR Mutation Detection Protocols: 688 (Methods in Molecular Biology, 688)

Featured Edition

ISBN 10:  1493956299 ISBN 13:  9781493956296
Publisher: Humana, 2016
Softcover