Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics: 131 (Methods in Molecular Medicine, 131) - Hardcover

Hardcover

ISBN 10: 1588299015 ISBN 13: 9781588299017

Publisher: Humana, 2007

Synopsis

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

About this title

Review

"...recommend it to investigators working in the area of viral-host interactions." -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars

 

Reviews of the First edition:

"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."- Microbiology Today

". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters

Synopsis

"Adenovirus Methods and Protocols" is designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. "Adenovirus Methods and Protocols, Volume II" focuses on methods that elucidate and quantitate the interactions of adenoviruses with the host. This volume provides methods for analysis of transcription, splicing, RNA interference, subcellular localization of proteins during infection, and cell cycle effects.

Edition details

• Publisher: Humana
• Publication date: 2007
• Language: English
• ISBN 10: 1588299015
• ISBN 13: 9781588299017
• Binding: Hardcover
• Edition number: 2
• Number of pages: 376
• Editor: Wold William S. M., Tollefson Ann E.