Agrobacterium Protocols: Volume II: 344 (Methods in Molecular Biology, 344) - Hardcover

Hardcover

ISBN 10: 1588298434 ISBN 13: 9781588298430

Publisher: Humana, 2006

Synopsis

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

About this title

Review

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species ... . the book will be a valuable addition to the laboratory bookshelf. The protocols ... are clearly set out and the notes explain things ... . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)

Synopsis

The second edition of "Agrobacterium Protocols" contains 80 chapters (two volumes) divided into 14 parts. In Volume II, there are a total of eight parts. Volume II contains seven of the eleven parts (Part VII to XIII) of plant protocols. Part XIV (Non-plants) contains six chapters with protocols for introducing DNA into non-plant species such as bacteria, fungi, algae, and mammalian cells. The description of this unique capacity of Agrobacterium is a new addition to this edition. This book provides a bench-top manual for tested protocols involving Agrobacterium-mediated transformation.

Edition details

• Publisher: Humana
• Publication date: 2006
• Language: English
• ISBN 10: 1588298434
• ISBN 13: 9781588298430
• Binding: Hardcover
• Edition number: 2
• Number of pages: 512
• Editor: Wang Kan