Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models: 130 (Methods in Molecular Medicine, 130) - Hardcover

Hardcover

ISBN 10: 1588295982 ISBN 13: 9781588295989

Publisher: Humana, 2007

Synopsis

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

About this title

Review

"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars

 

Reviews of the First edition:

"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today

". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters

Synopsis

"Adenovirus Methods and Protocols" is designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. The initial chapters of "Adenovirus Methods and Protocols, Volume 1" address several techniques for construction of adenoviruses for use as vectors and for basic research. The topics include deletion mutants, capsid modifications, insertions, and gene replacements both in human adenoviruses and murine, bovine, and ovine adenoviruses.

Edition details

• Publisher: Humana
• Publication date: 2007
• Language: English
• ISBN 10: 1588295982
• ISBN 13: 9781588295989
• Binding: Hardcover
• Edition number: 2
• Number of pages: 256
• Editor: Wold William S. M., Tollefson Ann E.