Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation (Current Clinical Pathology) - Hardcover

Nguyen, D.

Hardcover

ISBN 10: 1588292126 ISBN 13: 9781588292124

Publisher: Humana Press Inc., 2002

Synopsis

This unique text offers a systematic and practical approach to the analysis and interpretation of FCM graphics. Using numerous FCM illustrations derived from actual well-documented clinical cases, the authors demonstrate a step-by-step approach to optimal FCM data analysis on specimens suspected of harboring hematopoietic malignancies. The discussion moves from simple to complex specimens, with an emphasis on visual pattern analysis. A wide variety of hematologic disorders are covered, including leukemias and lymphomas. The companion CD-ROM with 80 detailed case studies provides additional opportunities to gain a deeper understanding of FCM data analysis.

About this title

Review

"Wow! This is really a nice book. There are only five chapters, but be forewarned - each chapter is quite comprehensive in its discussion of the subject matter. The text is factually dense and thorough. The opening two chapters logically and concisely lay out overall considerations for immunophenotyping -- the theoretical underpinnings, strengths and weaknesses of the technology, specimen considerations, how to decide what should be components of antibody panels, etc. I especially liked the brief but succinct and cautionary discussion of the lineage infidelity and limited clinical utility of various "diagnostic" cell surface markers. The third chapter eases the reader into the easiest category of studies to interpret - i.e., homogeneous specimens. The final two chapters delve into great depth on the analysis of heterogeneous specimens - those containing both normal and abnormal populations -- commonly encountered in the clinical setting and posing the greatest interpretative challenge. This is a really great book! It thoroughly summarizes all aspects of flow cytometry in the practice of hematopathology. I'm considering making it required reading for my residents."-Doody's Health Sciences Book Review Journal

From the Back Cover

Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematologic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. The authors include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis. The companion CD-ROM with 80 detailed case studies provides additional opportunities to gain a deeper understanding of FCM data analysis.
Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening of their FCM data analysis skills on a wide spectrum of hematologic disorders.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2002
• Language: English
• ISBN 10: 1588292126
• ISBN 13: 9781588292124
• Binding: Hardcover
• Number of pages: 256