Lentivirus Gene Engineering Protocols: v. 229 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 1588290913 ISBN 13: 9781588290915

Publisher: Humana Press Inc., 2003

Synopsis

Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.

About this title

Review

"The materials and methods are written in sufficient detail to allow even inexperienced workers to carry out experiments. However, the book would benefit from the inclusion of a section stating the sources of cloning and packaging plasmids. I would recommend this book to all who are planning to work with lentiviral vectors"-SGM Quarterly

From the Back Cover

Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2003
• Language: English
• ISBN 10: 1588290913
• ISBN 13: 9781588290915
• Binding: Hardcover
• Number of pages: 328
• Editor: Federico Maurizio