In Vitro Mutagenesis: Methods and Protocols: 1498 (Methods in Molecular Biology, 1498) - Softcover

 
9781493982110: In Vitro Mutagenesis: Methods and Protocols: 1498 (Methods in Molecular Biology, 1498)

Synopsis

In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

 

Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

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9781493964703: In Vitro Mutagenesis: Methods and Protocols: 1498 (Methods in Molecular Biology, 1498)

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ISBN 10:  1493964704 ISBN 13:  9781493964703
Publisher: Humana, 2016
Hardcover