Neurotrophic Factors: Methods and Protocols: 1727 (Methods in Molecular Biology, 1727) - Hardcover

 
9781493975709: Neurotrophic Factors: Methods and Protocols: 1727 (Methods in Molecular Biology, 1727)
Hardcover
ISBN 10: 1493975706 ISBN 13: 9781493975709
Publisher: Humana, 2017

Synopsis

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

"synopsis" may belong to another edition of this title.

From the Back Cover

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

"About this title" may belong to another edition of this title.

Publisher
Humana
Publication date
2017
Language
English
ISBN 10 
1493975706
ISBN 13 
9781493975709
Binding
Hardcover
Edition number
2
Number of pages
473
Editor
Skaper Stephen D.

Other Popular Editions of the Same Title

9781617795350: Neurotrophic Factors: Methods and Protocols: 846 (Methods in Molecular Biology, 846)

Featured Edition

ISBN 10:  1617795356 ISBN 13:  9781617795350
Publisher: Humana, 2012
Hardcover