Ribosome Display and Related Technologies: Methods and Protocols: 805 (Methods in Molecular Biology, 805) - Softcover

Softcover

ISBN 10: 1493958720 ISBN 13: 9781493958726

Publisher: Humana, 2016

Synopsis

Display technologies are a powerful biological tool, yet the complexity of ribosome display methods has inhibited their use. This volume provides detailed and easily followed protocols with the aim of facilitating a greater uptake of these methods.

About this title

From the Back Cover

Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols. The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.

Edition details

• Publisher: Humana
• Publication date: 2016
• Language: English
• ISBN 10: 1493958720
• ISBN 13: 9781493958726
• Binding: Paperback
• Number of pages: 437
• Editor: Douthwaite Julie A., Jackson Ronald H.