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Synopsis
This book fully updates the RT-PCR technologies presented in the popular first edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells and RT-PCR for cloning. In addition, this edition explores new technologies.
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Review
From the reviews of the second edition:
“The second edition of RT-PCR protocols and attempts to cover the most commonly used RT-PCR protocols, giving both beginners and experts a deeper understanding of the experimental set-up. ... There are often flow diagrams or pictures to aid understanding and the references are helpful, if you require additional information. ... a good investment for any lab interested in ensuring that their RT-PCR reactions are as productive and well-controlled as possible. ... Overall a useful and detailed text written in an informative and helpful style.” (Hayley Evans, Immunology News, May, 2011)From the Back Cover
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
"About this title" may belong to another edition of this title.
- PublisherHumana
- Publication date2016
- ISBN 10 1493956825
- ISBN 13 9781493956821
- BindingPaperback
- LanguageEnglish
- Number of pages352
- EditorKing Nicola
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RT-PCR Protocols: Second Edition (Methods in Molecular Biology, 630)
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RT-PCR Protocols: Second Edition (Methods in Molecular Biology, 630)
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RT-PCR Protocols
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression. 356 pp. Englisch. Seller Inventory # 9781493956821
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RT-PCR Protocols
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. -Features all the latest and hottest technology used in conventional and real-time RT-PCR-Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of complex cell populations-Serves as a comprehensive guid. Seller Inventory # 447956656
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RT-PCR Protocols : Second Edition
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Humana Press, Humana Press, 2016
ISBN 10: 1493956825
ISBN 13: 9781493956821
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression. Seller Inventory # 9781493956821
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RT-PCR Protocols: Second Edition
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ISBN 10: 1493956825
ISBN 13: 9781493956821
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Rt-pcr Protocols: Second Edition
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