Rab GTPases: Methods and Protocols: 1298 (Methods in Molecular Biology, 1298) - Hardcover

Hardcover

ISBN 10: 1493925687 ISBN 13: 9781493925681

Publisher: Humana, 2015

Synopsis

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.

About this title

About the Author

Guangpu Li, Ph.D. Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center 975 NE 10th Street, BRC 417B Oklahoma City, OK 73104 Tel: (405) 271-2227 ext. 61232 Fax: (405) 271-3910 E-mail: guangpu-li@ouhsc.edu

From the Back Cover

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters, and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling, and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.

Edition details

• Publisher: Humana
• Publication date: 2015
• Language: English
• ISBN 10: 1493925687
• ISBN 13: 9781493925681
• Binding: Hardcover
• Number of pages: 372
• Editor: Li Guangpu