1 The Enzyme Purification Laboratory.- 2 Making an Extract.- 3 Separation by Precipitation.- 4 Separation by Adsorption. I. General Principles.- 5 Separation by Adsorption. II. Specific Adsorbents.- 6 Separation in Solution.- 7 Optimization of Procedures; Recombinant Protein Techniques.- 8 Maintenance of Active Enzymes.- 9 Measurement of Enzyme Activity.- 10 Analysis for Purity: Crystallization.- Appendix A.- Addition of Precipitants.- Appendix B.- Solutions for Measuring Protein Concentration.- References.
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