Degrons: Methods and Protocols: 3069 (Methods in Molecular Biology, 3069) - Hardcover

Hardcover

ISBN 10: 1071655078 ISBN 13: 9781071655078

Publisher: Humana, 2026

Synopsis

This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function.

About this title

From the Back Cover

This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Degrons: Methods and Protocols serves as an ideal guide for researchers working on protein degradation or using degrons to study or control protein function.

Edition details

• Publisher: Humana
• Publication date: 2026
• Language: English
• ISBN 10: 1071655078
• ISBN 13: 9781071655078
• Binding: Hardcover
• Number of pages: 272
• Editor: Górna Maria W., Klimecka Maria M., Izert-Nowakowska Matylda A.