Germline Stem Cells: Methods and Protocols: 2677 (Methods in Molecular Biology, 2677) - Hardcover

Hardcover

ISBN 10: 1071632582 ISBN 13: 9781071632581

Publisher: Humana, 2023

Synopsis

This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Germline Stem Cells: Methods and Protocols, Third Edition is a valuable resource for all researchers in the germ stem cell community interested in learning more about this developing field.

About this title

From the Back Cover

This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Germline Stem Cells: Methods and Protocols, Third Edition is a valuable resource for all researchers inthe germ stem cell community interested in learning more about this developing field.

Edition details

• Publisher: Humana
• Publication date: 2023
• Language: English
• ISBN 10: 1071632582
• ISBN 13: 9781071632581
• Binding: Hardcover
• Edition number: 3
• Number of pages: 295
• Editor: Buszczak Michael