Chromatin Accessibility: Methods and Protocols: 2611 (Methods in Molecular Biology, 2611) - Hardcover

Book 237 of 381: Methods in Molecular Biology
 
9781071628980: Chromatin Accessibility: Methods and Protocols: 2611 (Methods in Molecular Biology, 2611)
Hardcover
ISBN 10: 1071628984 ISBN 13: 9781071628980
Publisher: Humana, 2023

Synopsis

This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and produce information about relative accessibility, the book continues with methods that use single-molecule and enzymatic approaches to solving the problem of mapping absolute occupancy/accessibility, emerging tools for mapping DNA accessibility and nucleosome positioning in single cells, imaging-based methods for visualizing accessible chromatin in its nuclear context, as well as computational methods for the processing and analysis of chromatin accessibility datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and up-to-date, Chromatin Accessibility: Methods and Protocols serves as an extensive and useful reference for researchers studying different facets of chromatin accessibility in a wide variety of biological contexts. 

Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

"synopsis" may belong to another edition of this title.

From the Back Cover

This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and produce information about relative accessibility, the book continues with methods that use single-molecule and enzymatic approaches to solving the problem of mapping absolute occupancy/accessibility, emerging tools for mapping DNA accessibility and nucleosome positioning in single cells, imaging-based methods for visualizing accessible chromatin in its nuclear context, as well as computational methods for the processing and analysis of chromatin accessibility datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and up-to-date, Chromatin Accessibility: Methods and Protocols serves as an extensive and useful reference for researchers studying different facets of chromatin accessibility in a wide variety of biological contexts. 

Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

"About this title" may belong to another edition of this title.

Publisher
Humana
Publication date
2023
Language
English
ISBN 10 
1071628984
ISBN 13 
9781071628980
Binding
Hardcover
Edition number
1
Number of pages
352
Editor
Marinov Georgi K., Greenleaf William J.

Other Popular Editions of the Same Title

9781071629017: Chromatin Accessibility: Methods and Protocols: 2611 (Methods in Molecular Biology, 2611)

Featured Edition

ISBN 10:  1071629018 ISBN 13:  9781071629017
Publisher: Humana, 2024
Softcover