The P2X7 Receptor: Methods and Protocols: 2510 (Methods in Molecular Biology, 2510) - Hardcover

Book 124 of 274: Methods in Molecular Biology
 
9781071623831: The P2X7 Receptor: Methods and Protocols: 2510 (Methods in Molecular Biology, 2510)
Hardcover
ISBN 10: 1071623834 ISBN 13: 9781071623831
Publisher: Humana, 2022

Synopsis

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.

"synopsis" may belong to another edition of this title.

From the Back Cover

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.

"About this title" may belong to another edition of this title.

Publisher
Humana
Publication date
2022
Language
English
ISBN 10 
1071623834
ISBN 13 
9781071623831
Binding
Hardcover
Edition number
1
Number of pages
408
Editor
Nicke Annette

Other Popular Editions of the Same Title

9781071623862: The P2X7 Receptor: Methods and Protocols: 2510 (Methods in Molecular Biology, 2510)

Featured Edition

ISBN 10:  1071623869 ISBN 13:  9781071623862
Publisher: Humana, 2023
Softcover