Protein Crystallization: Techniques, Strategies, and Tips (Iul Biotechnology Series) - Hardcover

Bergfors, Terese M

Hardcover

ISBN 10: 0963681753 ISBN 13: 9780963681751

Publisher: TBS The Book Service Ltd, 1999

Synopsis

This book is written for biochemists, crystallographers, graduate students, lab technicians, undergraduates or anyone else working with proteins with the intention of crystallizing them. The first part introduces the beginner to the basic techniques, materials, and parameters that affect crystallization. Those readers with previous crystallization experience can turn directly to the chapter on dynamic light scattering as a diagnostic tool, or to the five chapters devoted to choosing a crystallization strategy. Interpretation of results is probably the area in which many have their greatest difficulty so 20 color plates have been included to assist in the identification of the phenomena found in crystallization drops. The second part of the book takes up more advanced methods such as crystallization for cryo-crystallography, seeding, the use of oils, microbatch methods, and special considerations for membrane proteins. Useful tips and recommendations have been collected in an A-Z section, again with an emphasis on practical problems and questions encountered at the lab bench. The appendices contain a list of good-to-have gizmos, suppliers and sources of crystallization materials, useful web sites, and a comprehensive listing of commercially available screens Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The chapters have been contributed by 18 scientists who are actively involved in the development of methodology for protein crystallization.

About this title

Review

The chapters describe in a systematic manner, the means, tools, reagents, and procedures of protein crystallization -- Alexander McPherson, University of California

From the Back Cover

Completely revised and updated, the second edition of Protein Crystallization is a greatly expanded follow-up to the best-selling first edition. No previous background in crystallization is assumed. Contents include:

--New chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine labeling, rational strategies for crystallization, and protein modification to improve crystallization.

--Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals.

--Thirty full-color plates for evaluating crystallization drops.

--Separate section of laboratory exercises, ideal for crystallization courses.

--A-Z glossary.

Edition details

• Publisher: TBS The Book Service Ltd
• Publication date: 1999
• ISBN 10: 0963681753
• ISBN 13: 9780963681751
• Binding: Hardcover
• Language: English
• Number of pages: 320