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Synopsis
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
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From the Back Cover
In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented.
Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution.
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PCR Cloning Protocols
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PCR Cloning Protocols / Hrsg. Bing-Yuan Chen , Hrsg. Harry W. Janes
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Totowa : Humana Press, 2002
ISBN 10: 0896039730
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Condition: Gut. Ausgabe: 2., Ed., 2002 Umfang/Format: 460 Seiten , 229 mm x 152 mm Einbandart und Originalverkaufspreis: : EUR 85.55 (unverbindliche Preisempfehlung), sfr 131.00 (unverbindliche Preisempfehlung) 0-89603-973-0 : EUR 85.55 (unverbindliche Preisempfehlung), sfr 131.00 (unverbindliche Preisempfehlung) PCR Cloning Protocols, Second Edition, updates and expands Bruce White, s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA f Rutgers University, New. Brunswick From Reviews of the First Edition: .a balanced and easy to use guide to the principal contemporary options. Microbiology Today .eliminatles] mang laborious and expensive techniques associated with traditional Isolation and analysis. Biochemical Education In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes hy mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White, s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis an long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination essential methods for today, s study of functional genomics, gene expression, protein structure function relationships, protein engineering, and molecular evolution. Readily reproducible methods for isolating genes from all biological samples Cutting-edge techniques described by hands-on masters Azffiffleses Proven methods for cloning uncharacterized DNA z- flanking a known DNA fragment 111 Tested techniques for in vitro DNA recombination Part I. Performing and Optimizing PCR. Part II. Clon- bination. Part IV. Cloning Unknown Neighboring ing PCR Products. Part III. Mutagenesis and Recom- DNA. Part V. Library Construction and Screening. gutes Exemplar, ordentlich, Gern können sie Ihr Buch per Rechnung bestellen. Hardcover. Seller Inventory # B00070424
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