The Elisa Guidebook: 149 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896039501 ISBN 13: 9780896039506

Publisher: Humana Press Inc., 2000

Synopsis

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

About this title

Review

John R. Crowther has extensive experience in working with ELISA. The book reflects his xperience. The graphics and tables are extremely helpful and thoughtfully laid out. All of the ELISA systems are described both in the text and supported by graphical illustrations. Likewise, many of the tables regarding solving problems and troubleshooting are invaluable. Tables summarizing blocking buffers, definitions, and antibody combinations are helpful for quick reference...There is no other book of this kind currently in print. The ELISA Guidebook is essential to libraries at any health science research institution since use of this assay is commonplace across a wide variety of disciplines...This book would be extremely helpful at any immunodiagnostic company that develops ELISAs. Laboratories that create and use ELISA tests on a regular basis might find this book a useful purchase . - E-streams Electronic Book Review Journal

From the Back Cover

Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams.
With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2000
• ISBN 10: 0896039501
• ISBN 13: 9780896039506
• Binding: Hardcover
• Language: English
• Number of pages: 436
• Editor: Crowther John R.