The Protein Protocols Handbook (Methods in Molecular Biology) - Hardcover

Walker, John M

Hardcover

ISBN 10: 0896039404 ISBN 13: 9780896039407

Publisher: Humana Press Inc., 2002

Synopsis

This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the "Methods in Molecular Biology" series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls and on modifying the method to function within your own experimental circumstances.

About this title

Review

the breadth of techniques covered is large and this alone makes the book of practical value...The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory...It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins...This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory. - Doody's Health Sciences Book Review Journal Review of the first edition: The Protein Protocols Handbook has arrived at the right place at the right time. - Trends in Cell Biology From Reviews of the First Edition " a must for researchers in protein chemistry and especially those with less experience " - Doody's Health Sciences Book Review Journal " a very useful tool for daily work.." - Nahrung-Food " definitely an indispensable benchtop handbook highly recommended for all levels of research within the field." - Bioseparation " stands out in an already overcrowded field " - Aslib Book Guide This book is a must-have' for any laboratory involved in analysing proteins...it covers an astonishing range for a book. The chapters have been written by scientists with years of practical experience at the bench, whose enthusiasm for their subject oozes out of the pages. - Annals of Clinical Biochemistry

From the Back Cover

From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research
within the field."-Bioseparation
"...a very useful tool for daily work." -Nahrung-Food

Since publication of the bestselling first edition of John Walker's widely acclaimed Protein Protocols Handbook in 1996, there have been many highly significant developments in protein biochemistry. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2002
• Language: English
• ISBN 10: 0896039404
• ISBN 13: 9780896039407
• Binding: Hardcover
• Edition number: 2
• Number of pages: 1600