In Vitro Mutagenesis Protocols: v. 182 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896039102 ISBN 13: 9780896039100

Publisher: Humana Press Inc., 2001

Synopsis

Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in "In Vitro Mutagenesis Protocols, 2nd Edition" offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

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Review

"...strongly recommended to scientists who anticipate sophisticated exploitation of mutagenesis techniques in genetic engineering." - Folia Microbiologica

From the Back Cover

In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers. Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2001
• Language: English
• ISBN 10: 0896039102
• ISBN 13: 9780896039100
• Binding: Hardcover
• Edition number: 2
• Number of pages: 304
• Editor: Braman Jeff