A group of leading stem-cell investigators describe in detail the key laboratory methods for investigating hematopoietic stem cells. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and blood cancers such as leukemia.
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"Knowledge of hematopoietic stem cells is crucial in the development of clinical medicine, especially hematopoietic stem cell transplantation, solid organ transplantation, tissue engineering for the treatment of damaged tissue. Research in the area has made major advance in the past two decades. Various technologies were produced and contributed to the development. This book describes detailed techniques in each experiment as if authors are teaching to the investigators by their side. Each section also contains notes and introductions that explain practical tips, historical and logical aspects of those experiments. This book covers current important methodologies in hematopoietic stem cell research and helps investigators to perform correct techniques excellently. "- Doody's Health Sciences Book Review Journal. Numerical Score: 91 - 4 Stars!
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