In Situ Hybridization Protocols: No. 123 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896036863 ISBN 13: 9780896036864

Publisher: Humana Press Inc., 2000

Synopsis

Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques - many not covered in the first edition - and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.

About this title

Review

From Reviews of the First Edition by Andy Choo... "...a very helpful manual...essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire." - - Molecular Biotechnology " ...a good book for anyone wishing to exploit all that in situ hybridization has to offer." - - Biopharm Manufacturing Reviews for Second Edition This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both. - Heredity

From the Back Cover

In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2000
• Language: English
• ISBN 10: 0896036863
• ISBN 13: 9780896036864
• Binding: Hardcover
• Edition number: 2
• Number of pages: 368
• Editor: Darby Ian A.