RNA-Protein Interaction Protocols: v. 118 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896035689 ISBN 13: 9780896035683

Publisher: Humana Press Inc., 2010

Synopsis

Highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems. RNA Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that will assure productive and reproducible results for all investigators in the field.

About this title

Review

This manual is recommended to investigators with experience in molecular biological research, wishing to become introduced into the field of RNA-protein interactions. This book will also be a valuable source of reference for scientists needing general information about the theoretical basis and current methodological approaches to investigating ribonucleoprotein complexes. - International Journal of Medical Microbiology The coverage of the book is broad and reasonably comprehensive, with chapters arranged in a coherent sequence. Many of the authors are well-known, leading researchers in their field, and describe key contemporary techniques. In these respects the coverage is authoritative and likely to remain relevant for some years, before being overtaken by new technologies and methods represents value for money. - Cell Biology International This is a book which any scientist involved in any kind of cellular and molecular biology will find profitable for his experimental investigations since RNA and proteins are unavoidable matters to solve any problem in Life Sciences. Highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. - Cellular and Molecular Biology

From the Back Cover

Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2010
• Language: English
• ISBN 10: 0896035689
• ISBN 13: 9780896035683
• Binding: Hardcover
• Number of pages: 504
• Editor: Haynes Susan