MHC Protocols: 210 (Methods in Molecular Biology, 210) - Hardcover

Hardcover

ISBN 10: 0896035484 ISBN 13: 9780896035485

Publisher: Humana, 2002

Synopsis

Mhc Protocols Gives detail of readily reproducible methods for identifying polymorphisms in the human MHC. This work includes techniques that provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP 2, C2, C4, and TNF- a). Full description

About this title

From the Back Cover

Many of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease association and play a pivotal role in the control of immune response. In MHC Protocols, leading experts detail readily reproducible methods for identifying these polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP2, C2, C4, and TNF-a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is written by an investigator who has used the technique extensively and includes troubleshooting tips and notes on pitfalls to avoid.
Comprehensive and highly practical, MHC Protocols offers both experienced and novice investigators not only a state-of-the-art overview of this dramatically evolving area of immunology, but also a reliable guide to the identification of MHC gene polymorphisms.

Synopsis

A collection by leading experts detail of readily reproducible methods for identifying polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP 2, C2, C4, and TNF- a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles.

Edition details

• Publisher: Humana
• Publication date: 2002
• ISBN 10: 0896035484
• ISBN 13: 9780896035485
• Binding: Hardcover
• Language: English
• Number of pages: 352
• Editor: Powis Stephen H., Vaughan Robert W.