Animal Cell Biotechnology: Methods and Protocols: v. 8 (Methods in Biotechnology) - Hardcover

Hardcover

ISBN 10: 0896035476 ISBN 13: 9780896035478

Publisher: Humana Press Inc., 2010

Synopsis

Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

About this title

Review

A specific feature of cell culture technology is described in each chapter -- such as setting up a cell culture lab, production of large quantities of animal cells and cell products, large scale transfection protocols, and screening cultures for possible viral infection. There is an adequate number of illustrations to help the reader understand the nature of the equipment described and the expected data one should obtain. This book will be useful to individuals interested in the industrial or commercial applications and uses of animal cell culture methods. - Doody's Health Sciences Book Review Journal Animal Cell Biotechnology brings together techniques used in both industry and research laboratories that are focused on the expression of recombinant proteins and other commercial uses of animal cells. The book is divided into five parts describing techniques relevant for each step in this process. Detailed protocols are provided including recipes for culture media. In the last chapter of Part I, the problem of virus contamination of cell cultures used for the manufacture of therapeutic reagents is addressed and the subject is broadened to encompass viruses in both humans, laboratory animals and livestock. Overall, Part V very adequately prepares the reader for the next step which covers the molecular methods of the biotechnology. Part II Molecular methods', comprises six excellent chapters on gene expression optimization, immortalization strategies, production of monoclonal antibodies, cell bank preparation and cell type characterization, including cytogenetic characterization and DNA fingerprinting. - FEBS Letters

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2010
• Language: English
• ISBN 10: 0896035476
• ISBN 13: 9780896035478
• Binding: Hardcover
• Number of pages: 320
• Editor: Jenkins Nigel