Gene Isolation and Mapping Protocols: 68 (Methods in Molecular Biology, 68) - Hardcover

Hardcover

ISBN 10: 0896034828 ISBN 13: 9780896034822

Publisher: Humana, 1996

Synopsis

Over the past 10 years great progress has been made in the development of efficient techniques for both gene isolation and mapping. The identifica- tion and isolation of transcribed sequences from large chromosomal regions are central to the human genome mapping project. Techniques for isolating novel cDNAs have applications both in the overall construction and integra- tion of long-range physical and transcription maps and in the identification of disease genes. A number of different techniques for the isolation of cDNAs from mam- malian genomes have been developed, including screening "zoo" blots, the use of large genomic clones (YACs or cosmids) for hybridization against cDNA libraries, and CpG island mapping. More recently two highly efficient tech- niques have been introduced: exon trapping, based on the presence of exon splice sites, and direct selection, based on the enrichment of selected cDNAs using immobilized YACs or cosmids. Leading researchers in the field have contributed chapters detailing the practical procedures for these and other widely used methods. The most rapid progress presently being made in the field of gene isolation concerns the partial sequencing of cDNA clones from one or both ends to produce expressed sequence tags (ESTs). Indeed, by Octo- ber 1995, the EST division of Genbank (dbEST) contained a total of approxi- mately 270,000 human EST sequences accounting for almost half the number of sequence entries in Genbank.

About this title

Review

...a useful addition to the libraries of individuals and institutions... Three Stars. - Doody's Health Sciences Book Review Journal

"...this series of excellent and detailed lab manuals....of general use to all those mapping and isolation genes."-SGM Quarterly

Synopsis

An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Edition details

• Publisher: Humana
• Publication date: 1996
• ISBN 10: 0896034828
• ISBN 13: 9780896034822
• Binding: Hardcover
• Language: English
• Number of pages: 328
• Editor: Boultwood Jacqueline