Epitope Mapping Protocols: 66 (Methods in Molecular Biology, 66) - Softcover

Softcover

ISBN 10: 0896033759 ISBN 13: 9780896033757

Publisher: Humana, 1996

Synopsis

Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com­ prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first.

About this title

Synopsis

Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping - all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. A method for T-cell epitopes, instructions on using natural variants or mutants, and techniques for producing panels of antibodies for epitope mapping are also included.

Edition details

• Publisher: Humana
• Publication date: 1996
• Language: English
• ISBN 10: 0896033759
• ISBN 13: 9780896033757
• Binding: Paperback
• Number of pages: 431
• Editor: Morris Glenn E.