Natural Products Isolation (Methods in Molecular Biology) - Hardcover

Cannell, MGR

Hardcover

ISBN 10: 0896033627 ISBN 13: 9780896033627

Publisher: Humana Press Inc., 1998

Synopsis

Researchers from both industry and academia present in detail techniques for the extraction and isolation of natural products of all classes. These experienced experimentalists describe a wide array of methodologies, including basic chromatography, ion-exchange methods, HPLC, countercurrent and planar chromatography, SFE, and crystallization. They also address the specific problems involved in working with plants, marine organisms, water-soluble compounds, scale-up, dereplication and partial identification, and follow-up of a natural product lead. For the less-experienced they offer hands-on advice - using real examples - about how to approach an extraction in general, when and why to apply a particular technique, how to carry out the methods described, and how to modify conditions according to the compound being isolated.

About this title

Review

... offers extensive help in the selection of a suitable methodology...an excellent aid...closes a painfully evident gap in the selection of monographs on natural products chemistry...warmly recommended to anyone interested in the isolation of natural products. - Angewandte Chemie

From the Back Cover

Building on the high reputation of the first edition of Natural Products Isolation, the authors have in this second edition not only significantly updated all the techniques to reflect recent advances in the field, but also added three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. These readily reproducible methods make it possible for even the novice to extract, prepare, and isolate natural products from given source materials. The book includes not only powerful cutting-edge techniques, but also simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Natural Products Isolation, Second Edition, offers the aspiring natural products researcher all the information needed to attain mastery of essential methods and the more experienced investigator a useful reference guide to all the available techniques.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 1998
• Language: English
• ISBN 10: 0896033627
• ISBN 13: 9780896033627
• Binding: Hardcover
• Number of pages: 480