Flow Cytometry Protocols: v. 91 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896033546 ISBN 13: 9780896033542

Publisher: Humana Press Inc., 1997

Synopsis

Leading researchers and experimentalists update the field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and fluorescent in situ hybridization. Protocols for chromosome sorting and 5-6 color analysis are included as advanced methods. The techniques in this volume take advantage of the new instrumentation and probes that have recently been developed to advance the field of flow cytometry and improve standard methods.

About this title

Review

"...editors M.J. Jaroszeski and R. Heller succed in assembling a representative collection of protocols focused on structural and functional analysis of human and animal cells...is well arranged and produced."-Reviewed by J. Dolezel, Biologia Plantarum

From the Back Cover

In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 1997
• Language: English
• ISBN 10: 0896033546
• ISBN 13: 9780896033542
• Binding: Hardcover
• Number of pages: 288
• Editor: Jaroszeski Mark J., Heller R.