A timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing - all described by hands-on, masters of the procedures. Conveys valuable lessons and insights into using automated sequencers and mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acids by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. Also deals with such troublesome problems as identifying sites of modification and working with very small samples.
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This mint, unread, First Edition, 1st printing HARDBACK, Humana Press, New Jersey, 1996, has glossy covers. The book size is 6.25Ó w x 9.25Ó h with an index and 375 pristine acid-free pages. There are many diagrams. ISBN 0-89603-353-8.
This methodological guide can be recommended to all who are concerned with protein characterization or peptide mapping. - Reviewed by R. Moravec, Biologia Plantarum
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