In Vitro Mutagenesis Protocols: v. 57 (Methods in Molecular Biology) - Softcover

TROWER

Softcover

ISBN 10: 0896033325 ISBN 13: 9780896033320

Publisher: Humana Press Inc., 2009

Synopsis

In this volume experts from industrial and academic laboratories describe easily reproducible and up-to-date procedures for site directed and random mutagenesis. Site-directed protocols include those based on strand selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/strepavidin system. Many can be used with virtually any double-stranded DNA plasmid. The bookcovers genesis, linker scanning mutagenesis, nested deletion mutagenesis, and a specialized E coli mutator strain.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2009
• Language: English
• ISBN 10: 0896033325
• ISBN 13: 9780896033320
• Binding: Paperback
• Number of pages: 408