Plant Cell Electroporation And Electrofusion Protocols: 55 (Methods in Molecular Biology) - Softcover

Softcover

ISBN 10: 0896033287 ISBN 13: 9780896033283

Publisher: Humana Press Inc., 1995

Synopsis

Gene transfer is an essential technology for improving our under- standing of gene structure and function. Although there are many meth- ods by which DNA may be introduced into cells―including heat and chemical treatments, and microinjection―electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi- ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in- cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna- tives to electric field-based methods, citing the advantages and limita- tions of the various methods for achieving specific goals.

About this title

Synopsis

Leading experts and innovators describe in detail plant cell electroporation and electrofusion techniques for many types of plants. Their protocols cover a diverse set of plant cell types, including different cell types from a single organism (i.e., leaf cells and pollen) and the most important model plants, including maize and tobacco. Each protocol also contains detailed instructions for growth and growth optimization, protoplast generation, and protoplast regeneration for specific plant cell types, along with extensive troubleshooting advice and descriptions of expected results to simplify study design.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 1995
• Language: English
• ISBN 10: 0896033287
• ISBN 13: 9780896033283
• Binding: Spiral-bound
• Number of pages: 206
• Editor: Nickoloff Jac A.