Capillary Electrophoresis Guidebook: Principles, Operation, and Applications: 52 (Methods in Molecular Biology, 52) - Hardcover

Altria, Kevin D.

Hardcover

ISBN 10: 0896033155 ISBN 13: 9780896033153

Publisher: Humana, 1995

Synopsis

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

About this title

Review

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE.-FEBS Letters

Synopsis

"Capillary Electrophoresis Guidebook" offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.

Edition details

• Publisher: Humana
• Publication date: 1995
• Language: English
• ISBN 10: 0896033155
• ISBN 13: 9780896033153
• Binding: Hardcover
• Number of pages: 358